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Michael C. Kolios, PhD

VIC-733
350 Victoria Street
Toronto, Ontario M5B 2K3
416-979-5000 x3157
mkolios@ryerson.ca

Specialization

  • Ultrasound imaging and therapeutics
  • Ultrasound imaging
  • Optical Coherence Tomography
  • Photoacoustics
  • Theranostic agent development
  • Heat transfer in tissue
  • Thermal Therapies

Research

My research interests are in the application of ultrasound in medicine and the modeling of heat transfer in tissues. I am currently examining the potential of using high frequency ultrasound imaging (20-60MHz) for the detection of cell death. Even though at these frequencies we cannot resolve individual cells, we can examine the ultrasound backscatter from cell ensembles to make inferences about the state of the cells. We have found that the ultrasound backscatter from cells dying either by apoptosis (a form of cell suicide) or necrosis increases, and the frequency dependence of the backscatter power spectra can potentially be used to differentiate between the two, at least in the in vitro systems we have used to date. I am collaborating with researchers at the Sunybrook Hospital in Toronto to determine whether this methodology can also be used in animal tumor models (to detect the death of cancer cells), and we are currently participating in a clinical trial involving cancer patients with superficial tumors. We have developed theoretical models to better understand the backscattering process, and have used experimental systems to examine the backscatter from objects on the scale of cells for which we know their properties. Ultrasonic characterization of cells and cellular components is thus an ongoing interest in the lab. Ultrasound at higher intensities can be used to destroy tissues rather than image them. We have developed theoretical models to estimate temperatures during the heating of tissue using ultrasound or other sources (such as laser), and are currently examining the effect of blood flow on these heating patterns. Furthermore I am investigating the use of an acoustic camera to monitor the treated tissue non-invasively. The acoustic camera records how ultrasound is attenuated rather than detecting the backscatter as conventional ultrasound does. Finally, I have incorporated in my research program optical techniques as a source of new contrast: optical coherence tomography and photoacoustic imaging, which currently form a substantial core of my research program.

Publications

Spatial interference encoding patterns based photoacoustic microscopy
2017; Optics Communications; Meiri, A. | Strohm, E.M. | Kolios, M.C. | Zalevsky, Z.

High frequency ultrasound imaging and simulations of sea urchin oocytes
2017; Journal of the Acoustical Society of America; Strohm, E.M. | Wirtzfeld, L.A. | Kolios, M.C. | Czarnota, G.J.

A magnetic droplet vaporization approach using perfluorohexane-encapsulated magnetic mesoporous particles for ultrasound imaging and tumor ablation
2017; Biomaterials; Teng, Z. | Wang, R. | Zhou, Y. | Kolios, M. | Wang, Y. |...

Monitoring Quantitative Ultrasound Parameter Changes in a Cell Pellet Model of Cell Starvation
2017; Biophysical Journal; Wirtzfeld, L.A. | Berndl, E.S.L. | Czarnota, G.J. | Kolios, M.C.

Stable microfluidic flow focusing using hydrostatics
2017; Biomicrofluidics; Gnyawali, V. | Saremi, M. | Kolios, M.C. | Tsai, S.S.H.

Erratum to: Assessment of the Nucleus-to-Cytoplasmic Ratio in MCF-7 Cells Using Ultra-high Frequency Ultrasound and Photoacoustics (Int J Thermophys, (2016), 37, (118), 10.1007/s10765-016-2129-y)
2017; International Journal of Thermophysics; Moore, M.J. | Strohm, E.M. | Kolios, M.C.

Low-power noncontact photoacoustic microscope for bioimaging applications
2017; Journal of Biomedical Optics; Sathiyamoorthy, K. | Strohm, E.M. | Kolios, M.C.

Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation
2017; Photoacoustics; Hysi, E. | Wirtzfeld, L.A. | May, J.P. | Undzys, E. | Li, S.-D. |...

Cancer treatment response evaluation using photoacoustic signal envelop statistics: A preliminary study
2017; 2016 International Conference on Bio-Engineering for Smart Technologies, BioSMART 2016; Hussein, S. | Falou, O. | Nasr, R. | Hysi, E. | Wirtzfeld, L.A. |...

Ultrasound spectral analysis of photoacoustic signals from red blood cell populations at different optical wavelengths
2017; Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Fadhel, M.N. | Kolios, M.C.

Multispectral photoacoustic bioimaging using low power continuous wave lasers
2017; Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Sathiyamoorthy, K. | Strohm, E.M. | Kolios, M.C.

Spatial interference encoding patterns based super resolved photoacoustic microscopy
2017; Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Meiri, A. | Strohm, E.M. | Kolios, M.C. | Zalevsky, Z.

Quantitative photoacoustic assessment of red blood cell aggregation under pulsatile blood flow: Experimental and theoretical approaches
2017; Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Bok, T.-H. | Hysi, E. | Kolios, M.C.

Ultrasound imaging of DNA-damage effects in live cultured cells and in brain tissue
2017; Methods in Molecular Biology; Tadayyon, H. | Gangeh, M.J. | Vlad, R. | Kolios, M.C. | Czarnota, G.J.

Ultrasound imaging of apoptosis: Spectroscopic detection of DNA-damage effects in vivo
2017; Methods in Molecular Biology; Tadayyon, H. | Gangeh, M.J. | Vlad, R. | Kolios, M.C. | Czarnota, G.J.